In Vitro Ultramorphological Assessment of Apoptosis on CEMss Induced by Linoleic Acid-Rich Fraction from Typhonium flagelliforme Tuber
Percentages of viable, early apoptotic, late apoptosis and secondary necrotic cells after DCM/F7 treatment. CEMss cell death via apoptosis increased significantly () in a time-dependent manner. However, no significant () difference was observed in the cell count of necrosis. Cells were cultured in RPMI 1640 (25 ml flask) media maintained at 37°C and 5% CO2. EA: Early apoptosis; LA: Late apoptosis; SN: Secondary necrosis.