Synergistic Antibacterial Effects of Polyphenolic Compounds from Olive Mill Wastewater

Table 1

Retention time and calculated concentrations of the different compounds isolated and identified from 1000 ppm AntiSolvent extract of OMW obtained by HPLC-PAD. The results are the mean of 3 replicates and based on the calculation of the area of injected standards. The concentration was calculated as area/slope of each peak and presented as means ± SD.

Compound	Retention time (min)	Area	Slope	𝑅^2	Concentration (ppm)
Hydroxytyrosol	7.105	12838384	124745	0.9857	102.9±1.1
3,4-dihydroxyphenylacetic acid	8.865	2103719	203297	0.9959	10.3±0.4
Tyrosol	10.775	2274965	87195	0.9660	26.1±0.2
Protocatechuic acid	11.315	1620535	118665	0.9877	13.7±0.1
Verbascoside	11.793	2295010	31060	0.9854	73.9±0.6
Vanillic acid	12.155	3349562	274065	0.9901	12.2±0.3
Caffeic acid	12.750	2029414	277494	0.9922	7.3±0.3
Ferulic acid	14.723	4003895	254636	0.9495	15.7±0.3
p-coumaric acid	15.463	3448623	241817	0.9990	14.3±0.3