Table of Contents Author Guidelines Submit a Manuscript
Evidence-Based Complementary and Alternative Medicine
Volume 2011 (2011), Article ID 459531, 8 pages
http://dx.doi.org/10.1155/2011/459531
Research Article

A Chinese Herbal Decoction, Modified Yi Guan Jian, Induces Apoptosis in Hepatic Stellate Cells through an ROS-Mediated Mitochondrial/Caspase Pathway

1School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung 404, Taiwan
2Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Taoyuan 333, Taiwan
3Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404, Taiwan
4Department of Life Sciences, National Chung Hsing University, Taichung 402, Taiwan

Received 9 April 2010; Revised 12 August 2010; Accepted 16 August 2010

Copyright © 2011 Hung-Jen Lin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Representative HPLC chromatograms of modified Yi Guan Jian. The Hitachi HPLC system equipped with a UV-VIS detector was used to analyze the extract of mYGJ. The amount of samples injected was 10 μl of mYGJ extract which was dissolved in 10 ml of methanol and applied to ultrasound shock for 60 min. The 0.45 μm membrane filtrated specimen was used to examine by HPLC. For Ferulic acid, the column was a 5 μm Mightysil RP-18 GP (4.6 mm × 250 mm). The mobile phase was a gradient elution of 0.03% H3PO4 (A) and acetonitrile (B), commencing with 10% B for 10 min and then a linear gradient to 90% B was applied over 45 min. Flow rate was 1.0 ml/min. The detector was set at wavelength 320 nm. For Catalpol, the column was a Waters Cosmosil 5NH2-MS (4.6 mm× 250 mm). The mobile phase was eluted by acetonitrile over 75 min. Flow rate was 1.0 ml/min. The detector was set at wavelength 203 nm.

  1. Supplementary Figure