Cranberry and Grape Seed Extracts Inhibit the Proliferative Phenotype of Oral Squamous Cell Carcinomas
Figure 10
Total RNA and mRNA analysis. RT-PCR was performed on total RNA extracted from CAL27 (a) and SCC25 (b) cells at 24 h after CE or GSE administration; no significant changes were detected at 4 h (data not shown). Relative endpoint (RE) RT-PCR revealed that CE and GSE treatment increased expression of apoptosis-related mRNA (caspase-2, caspase-8) in both cell lines, which is first detectable at 24 h. CE significantly enhanced CAL27 expression of cell-cycle genes (p53, c-myc, ODC), while GSE reduced SCC25 expression of these same targets at this time point.
