Anti-Inflammatory Potential of Ethanolic Leaf Extract of Eupatorium adenophorum Spreng. Through Alteration in Production of TNF-α, ROS and Expression of Certain Genes

Table 1

Primers.

Primers	Accession number	Sense (5′–3′)	Antisense (3′–5′)	𝑇m (°C)
IKK	NM_010546	CCAGACTCCAAGGTGGTGTT	TGCAGATCACAGGCAGAAAC	60.0
TNF-𝛼	NM_013693	TGGCACAGCCAAG	GGGACCCCTGCTC	52.36
TGF-𝛽	NM_011577	TTGCTTCAGCTCCACAGAGA	TGGTTGTAGAGGGCAAGGAC	59.99
IL-1𝛽	NM_008361	GTGGCAGCTACCTGTGTCTT	GGAGCCTGTAGTGCAGTTGT	57.96
IL-6	NM_031168	GGGAAATCGTGGA	AGGTTTGCCGAGT	43.9
IL-10	NM_010548	CCAAGCCTTATCGGAAATGA	TTTTCACAGGGGAGAAATCG	60.035
COX1	BC023322	AGAAACTGGTCTGCCTCA	AACCCACATCAAGGACTG	54.02
COX2	NM_011198	AGCACCATTCTCCTTGAA	GTAGGCTGTGGATCTTGC	54.0
PKC-𝜃	NM_008859	AAGTGAGAAACCCCGGCTAT	AGGCAAATCCCTTCCAGTCT	60.01
Perforin	NM_011073	ACCCTGAATGGGCTCACA	GCAGCAGTCCTGGTTGGT	57.0