REC-2006—A Fractionated Extract of Podophyllum hexandrum Protects Cellular DNA from Radiation-Induced Damage by Reducing the Initial Damage and Enhancing Its Repair In Vivo
Figure 2
(a) Effect of REC-2006 on 10 Gy-induced single strand breaks in mouse thymocytes. Mice were irradiated with or without REC-2006 treatment (8 mg k body weight i.p. 60 minutes before irradiation) and thymocytes from them were isolated at different intervals. DNA damage was studied employing the alkaline halo assay. (a) Cells without any treatment. (b–d) Cells from 10 Gy irradiated mice killed after 0, 15 or 60 min, respectively. (e–g) Cells from REC-2006 pretreated irradiated mice killed after 0, 15 or 60 min, respectively. (b) Quantitative estimation of REC-2006 effect on radiation-induced DNA damage and repair. The level of DNA strand breakage was quantified by calculating the nuclear spreading factor value, which is the ratio of the area occupied by the halo (obtained by subtracting the area of the nucleus from the total area of the nucleus + halo) to the area occupied by the nucleus. Data are expressed as the relative nuclear spreading factor (RNSF), which was calculated by subtracting the nuclear spreading factor values of control cells from those of treated cells. Data are the mean ± SD for at least 100 cells for each observation in triplicate.
