REC-2006—A Fractionated Extract of Podophyllum hexandrum Protects Cellular DNA from Radiation-Induced Damage by Reducing the Initial Damage and Enhancing Its Repair In Vivo
Figure 4
Effect of REC-2006 on radiation-induced hydroxyl generation and subsequent degradation of 2-deoxyribose, as measured by percentage inhibition in the formation of TBARS. Briefly, 1 ml of reaction volume containing, 5 mM 2-deoxyribose and varied concentrations of REC-2006 were mixed either with 100 M FeS(Fenton) or exposed to 100 Gy followed by incubation for 1 h at 37°C. There after, two volumes of solution containing 25 TCA and 1 TBA in 0.1 N NaOH was added, incubated in boiling water bath for 20 min, cooled and the absorbance of the resulting pink-colored chromogen was measured at 532 nm. The data in this figure represent the mean ± SD of values from three independent assays (*P .05, **P .01).
