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Evidence-Based Complementary and Alternative Medicine
Volume 2011 (2011), Article ID 478246, 8 pages
Original Article

Analgesic Effects and the Mechanisms of Anti-Inflammation of Hispolon in Mice

1Institute of Chinese Pharmaceutical Sciences, Taichung 40402, Taiwan
2School of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan
3Department of Pesticide Toxicology, Taiwan Agricultural Chemicals and Toxic substances Research institute, Wu Feng, Taichung 433, Taiwan

Received 29 October 2008; Accepted 3 March 2009

Copyright © 2011 Heng-Yuan Chang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Hispolon, an active ingredient in the fungi Phellinus linteus was evaluated with analgesic and anti-inflammatory effects. Treatment of male ICR mice with hispolon (10 and 20 mg/kg) significantly inhibited the numbers of acetic acid-induced writhing response. Also, our result showed that hispolon (20 mg/kg) significantly inhibited the formalin-induced pain in the later phase (P<.01). In the anti-inflammatory test, hispolon (20 mg/kg) decreased the paw edema at the fourth and fifth hour after λ-carrageenin (Carr) administration, and increased the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GRx) in the liver tissue. We also demonstrated that hispolon significantly attenuated the malondialdehyde (MDA) level in the edema paw at the fifth hour after Carr injection. Hispolon (10 and 20 mg/kg) decreased the nitric oxide (NO) levels on both the edema paw and serum level at the fifth hour after Carr injection. Also, hispolon (10 and 20 mg/kg) diminished the serum TNF-α at the fifth hour after Carr injection. The anti-inflammatory mechanisms of hispolon might be related to the decrease in the level of MDA in the edema paw by increasing the activities of SOD, GPx and GRx in the liver. It probably exerts anti-inflammatory effects through the suppression of TNF-α and NO.