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Evidence-Based Complementary and Alternative Medicine
Volume 2011, Article ID 485471, 9 pages
http://dx.doi.org/10.1093/ecam/neq066
Original Article

Ameliorate Effects of Li-Fu Formula on IL-6-Mediated Cardiac Hypertrophy in Hamsters Fed with a Hyper-Cholesterol Diet

1Emergency Department of Taichung Veterans General Hospital, China Medical University, Taiwan
2Department of Biological Science and Technology, China Medical University, Taiwan
3Graduate Institute of Basic Medical Science, China Medical University, Taiwan
4Department of Food and Nutrition, Huang-Kuang University, Taiwan
5Graduate Institute of Chinese Medical Science, China Medical University, Taiwan
6Department of Pediatrics, Medical Research and Medical Genetics, China Medical College Hospital, Taiwan
7Department of Healthcare Administration, Asia University, Taiwan
8Department of Health and Nutrition Biotechnology, Asia University, Taiwan
9Institute of Immunology, School of Medicine, Chung Shan Medical University, Taichung, Taiwan No. 110, Sec. 1, Jianguo N. Road, Taichung 402, Taiwan
10Clinical Laboratory, School of Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan. No. 110, Sec. 1, Jianguo N. Road, Taichung 402, Taiwan
11Department of Biochemistry, School of Medicine, Chung Shan Medical University, Taichung, Taiwan. No. 110, Sec. 1, Jianguo N. Road, Taichung 402, Taiwan
12Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung, Taiwan. No. 110, Sec. 1, Jianguo N. Road, Taichung 402, Taiwan

Received 11 March 2010; Accepted 13 May 2010

Copyright © 2011 Yi-Chang Cheng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Hypercholesterolemia diets are considered as major sources to cause cardiac hypertrophy. This study intends to evaluate the effects of Li-Fu formula on cardiac hypertrophy induced by hypercholesterolemia diet. Twenty-four male Golden Syrian hamsters were randomly divided into control, cholesterol and Li-Fu formula groups and fed with different experimental diets for 2 months. Histopathological analysis and western blotting were performed to measure the myocardial architecture, and various cardiac hypertrophy-associated molecules in the excised left ventricle from hamsters. The ratios of whole heart weight/body weight (BW) and left ventricle weight/BW were significantly higher in the cholesterol group but significantly lower in the Li-Fu formula group. The protein levels of both atrial natriuretic peptide and brain natriuretic peptide were significantly increased in the cholesterol group but significantly reduced in the Li-Fu formula group. Additionally, significantly increased interleukin-6, STAT3, MEK5, p-ERK5 and non-cardiomyocyte proliferate signal molecules such as p-MEK and p-ERK, were detected in the cholesterol group but significantly reduced in the Li-Fu formula group. Notably, no significant variations of inflammatory signaling molecules, including p-P38 and p-JNK, were detected in all groups. Our experimental results demonstrated the significant reductions of cardiac hypertrophy and related eccentric hypertrophy signaling, non-cardiomyocyte proliferate signaling in the excised left ventricle of hamsters from the Li-Fu formula. We suggested the protective effects of Li-Fu formula on cardiac hypertrophy that may be useful in prevention or treatment of hypertrophy-associated cardiovascular diseases.