Antioxidant and Preventive Effects of Extract from Nymphaea candida Flower on In Vitro Immunological Liver Injury of Rat Primary Hepatocyte Cultures

Table 3

Scavenging of, superoxide (O₂⁻), hydroxyl radical (·OH) and hydrogen peroxide (H₂O₂) by NCE.

Group	Concentration	O2^−		·HO		H2O2
	(𝜇g/mL)	Inhibition (%)	IC50 (𝜇g/mL)	Inhibition (%)	IC50 (𝜇g/mL)	Inhibition (%)	IC50 (𝜇g/mL)
Gallic acid	2	9.05 ± 0.10		30.56 ± 1.63		11.12 ± 1.12
	10	16.95 ± 0.34	29.39	43.2 ± 0.53	16.29	20.8 ± 1.54	31.87
	50	68.72 ± 0.25		62.14 ± 0.74		63.7 ± 1.28
BHT	2	5.57 ± 0.47		20.85 ± 1.02		4.42 ± 0.12
	10	23.12 ± 1.32	71.87	31.2 ± 0.44	83.56	18.29 ± 0.29	131.6
	50	39.83 ± 1.21		45.69 ± 1.89		30.43 ± 0.31
NCE	10	11.6 ± 0.70		25.43 ± 0.22		19.02 ± 0.64
	20	25.01 ± 1.01	59.32	38.22 ± 0.96	24.28	27.22 ± 1.49	86.45
	50	44.16 ± 0.43		59.02 ± 1.16		40.91 ± 1.26

IC₅₀, value of the 50% inhibition concentration; inhibition (%), percent inhibition of means of six replicates from the control.