Antioxidant and Preventive Effects of Extract from Nymphaea candida Flower on In Vitro Immunological Liver Injury of Rat Primary Hepatocyte Cultures

Table 5

Effect of NCE on supernatant AST levels in immunologic injury in primary cultured rat hepatocytes in vitro.

Group	Dose	AST (U/L)
	𝜇g/mL	3 hours	6 hours	12 hours	24 hours
Control	—	21.45 ± 2.92	24.17 ± 3.21	40.87 ± 6.01	41.00 ± 8.07
BCG + LPS	—	57.03 ± 2.88^a	90.55 ± 10.46^a	101.88 ± 10.71^a	63.34 ± 8.90^a
BCG + LPS + Grz	5	65.15 ± 16.64	59.89 ± 8.66^b	73.84 ± 10.89^b	37.67 ± 6.16^b
	10	48.53 ± 10.34	43.55 ± 12.02^b	60.66 ± 6.05^b	41.12 ± 16.37^b
	20	57.34 ± 9.57	59.68 ± 16.59^b	55.06 ± 8.17^b,c	33.38 ± 7.92^b
BCG + LPS + NCE	5	67.78 ± 6.41	53.79 ± 8.83^b	58.66 ± 4.72^b	37.76 ± 3.01^b
	20	56.88 ± 13.46	55.55 ± 7.20^b,d	48.72 ± 2.10^b	35.43 ± 4.34^b
	80	57.65 ± 6.24	36.71 ± 10.00^b,d	49.94 ± 6.25^b	35.63 ± 10.00^b

Values are the mean ± SD, 𝑛=6. ^a𝑃<.01 compared with control group; ^b𝑃<.01 compared with BCG + LPS group; ^c𝑃<.05 compared with BCG + LPS + Grz group (5 𝜇g/mL); ^d𝑃<.05 compared with BCG + LPS + NCE group (5 𝜇g/mL).