Antioxidant and Preventive Effects of Extract from Nymphaea candida Flower on In Vitro Immunological Liver Injury of Rat Primary Hepatocyte Cultures

Table 7

Effect of NCE on supernatant NO production in immunologic injury in primary cultured rat hepatocytes in vitro.

Group	Dose	NO (𝜇mol/l)
	𝜇g/mL	3 hours	6 hours	12 hours	24 hours
Control	—	2.38 ± 0.19	2.52 ± 0.18	2.80 ± 0.21	2.82 ± 0.21
BCG + LPS	—	5.03 ± 0.33^a	8.51 ± 0.49^a	10.57 ± 0.77^a	7.41 ± 0.77^a
BCG + LPS + Grz	5	4.96 ± 0.31	6.49 ± 0.74^b	8.13 ± 0.55^b	5.73 ± 0.64^b
	10	4.25 ± 0.43^b,c	5.83 ± 0.48^b,c	7.65 ± 0.55^b,c	4.84 ± 0.4^b,d
	20	3.63 ± 0.38^b,c	4.83 ± 0.55^b,c	6.08 ± 0.39^b,c	4.64 ± 0.51^b,c
BCG + LPS + NCE	5	5.10 ± 0.13	6.41 ± 0.73^b	7.89 ± 0.39^b	5.87 ± 0.45^b
	20	4.53 ± 0.73	5.84 ± 0.51^b	7.41 ± 0.71^b	5.50 ± 0.58^b
	80	4.44 ± 0.7	5.07 ± 0.60^b,e	6.69 ± 0.39^b,e	5.08 ± 0.69^b,f

Values are the mean ± SD, 𝑛=6. ^a𝑃<.01 compared with control group; ^b𝑃<.01 compared with BCG + LPS group; ^c𝑃<.05; ^d𝑃<.01 compared with BCG + LPS + Grz group (5 𝜇g/mL); ^e𝑃<.05 and ^f𝑃<.01 compared with BCG + LPS + NCE group (5 𝜇g/mL).