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Evidence-Based Complementary and Alternative Medicine
Volume 2011, Article ID 515647, 10 pages
http://dx.doi.org/10.1155/2011/515647
Research Article

Evaluation of Traditional Indian Antidiabetic Medicinal Plants for Human Pancreatic Amylase Inhibitory Effect In Vitro

1Institute of Bioinformatics and Biotechnology, University of Pune, Ganeshkhind, Pune 411007, India
2School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam, Kerala 690525, India
3Department of Zoology, Molecular Embryology Laboratory, University of Pune, Pune 411007, India

Received 19 May 2010; Accepted 7 August 2010

Copyright © 2011 Sudha Ponnusamy et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Pancreatic α-amylase inhibitors offer an effective strategy to lower the levels of post prandial hyperglycemia via control of starch breakdown. Eleven Ayurvedic Indian medicinal plants with known hypoglycemic properties were subjected to sequential solvent extraction and tested for α-amylase inhibition, in order to assess and evaluate their inhibitory potential on pancreatic α-amylase. Analysis of 91 extracts, showed that 10 exhibited strong Human Pancreatic Amylase (HPA) inhibitory potential. Of these, 6 extracts showed concentration dependent inhibition with IC50 values, namely, cold and hot water extracts from Ficus bengalensis bark (4.4 and 125 μgmL-1), Syzygium cumini seeds (42.1 and 4.1 μgmL-1), isopropanol extracts of Cinnamomum verum leaves (1.0 μgmL-1) and Curcuma longa rhizome (0.16 μgmL-1). The other 4 extracts exhibited concentration independent inhibition, namely, methanol extract of Bixa orellana leaves (49 μgmL-1), isopropanol extract from Murraya koenigii leaves (127 μgmL-1), acetone extracts from C. longa rhizome (7.4 μgmL-1) and Tribulus terrestris seeds (511 μgmL-1). Thus, the probable mechanism of action of the above fractions is due to their inhibitory action on HPA, thereby reducing the rate of starch hydrolysis leading to lowered glucose levels. Phytochemical analysis revealed the presence of alkaloids, proteins, tannins, cardiac glycosides, flavonoids, saponins and steroids as probable inhibitory compounds.