Emodin Induces Apoptotic Death in Murine Myelomonocytic Leukemia WEHI-3 Cells In Vitro and Enhances Phagocytosis in Leukemia Mice In Vivo
Figure 5
Emodin affected the apoptosis-associated protein levels in WEHI-3 cells. Cells were treated with emodin at 100 μM for 0, 6, 12, and 24 h, and then the total proteins were prepared and determined as described in Section 2. The levels of associated proteins expressions ((a): caspase-3, -7, and -9 and PARP; (b): cytochrome c, Apaf-1, AIF and Endo G; (c): GADD153, GRP78, ATF-6α and caspase-12; (d): Bcl-2, Bcl-xL, Bax, and Bad) were estimated by Western blotting as described in Materials and Methods.
