Oral Treatment with Extract of Agaricus blazei Murill Enhanced Th1 Response through Intestinal Epithelial Cells and Suppressed OVA-Sensitized Allergy in Mice

Figure 1

In vitro intestinal gut model constructed with Caco-2 cells in apical compartment (a) and RAW264.7 cells in basolateral compartment (b). Transwell inserts on which Caco-2 cells had been cultured were inserted into multiple plate wells containing RAW264.7 cells. Mineral oil (50 𝜇l/well) was added to the surface of the apical compartment (a) to mimic anaerobic conditions.