Oral Treatment with Extract of Agaricus blazei Murill Enhanced Th1 Response through Intestinal Epithelial Cells and Suppressed OVA-Sensitized Allergy in Mice
Figure 6
Effect of ABME on INF- and TNF- production from OVA-stimulated splenocytes prepared from OT-II mice via Caco-2 cells using a coculture system. Splenocytes were cultured in 24-wells plates for 2 h in 5% CO2 and incubated at to precipitate cells on the bottom of the plates. Then, the transwell inserts on which the Caco-2 cells had been cultured were placed into the 24 well plates, which had been preloaded with splenocytes. Two hundred microliters of ABME (250 g/ml) were applied to the apical side and incubated for 3h. After incubation, the inserts were removed, and the splenocytes were restimulated with 10 g/ml OVA at in a CO2 incubator for 72 h. After incubation, the culture media were collected for the measurement of IFN- (a) and TNF- (b) contents as described in Materials and Methods. Values represent the means ± SE. (). , significantly different from the values of the control group.
