Oral Treatment with Extract of Agaricus blazei Murill Enhanced Th1 Response through Intestinal Epithelial Cells and Suppressed OVA-Sensitized Allergy in Mice
Figure 7
TNF- and NO production in the coculture system or RAW264.7 cells alone treated with ABME or LPS ABME or LPS was added into the apical compartment of the Caco-2/RAW264.7 coculture system (a, c) or added directly into the RAW264.7 cells alone (b, d) and was then incubated for 12 h. After incubation, the supernatants were collected. TNF- and NO secretion into the culture supernatant (basolateral compartment) was determined by a cytotoxicity assay and Griess reagent. Values represent the means ± SE. (). , significantly different from the values of the control group.
