Gastroprotective Effect of Rubia cordifolia Linn. on Aspirin Plus Pylorus-Ligated Ulcer

Table 1

Effect of R. cordifolia on gastric acid secretion and ulcer index.

Sr. No.

1

2

3

4

5

6

7

8

9

10

11

12

Group

Normal

Control

Standard

ME 100

ME 200

ME 400

CF 50

CF 100

CF 200

RF 50

RF 100

RF 200

Ulcer Index (%protection)

—

80.63±3.88

21.88±2.01^∗∗∗ (72.86)

68.82±2.22^NS (14.64)

66.24±4.48^NS (17.84)

54.42±3.54^∗∗ (32.50)

52.39±1.51^∗∗ (35.02)

40.82±3.87^∗∗∗ (49.37)

30.87±2.24^∗∗∗ (61.71)

72.89±3.18^NS (9.59)

70.82±2.26^NS (12.16)

69.65±2.15^NS (13.61)

Pepsin (nmols of tyrosine/mL)

—

21.63±1.21

4.62±1.03^∗∗∗

15.85±1.62^NS

13.32±1.41^∗∗

11.82±1.02^∗∗∗

13.82±1.39^∗∗

11.28±1.22^∗∗∗

10.62±1.36^∗∗∗

17.27±0.71^NS

15.62±1.66^NS

14.27±2.19^∗

Protein (μg/mL)

—

174.47±9.03

99.42±2.61^∗∗∗

148.26±5.38^∗

131.27±1.63^∗∗∗

129.53±2.82^∗∗∗

130.62±2.11^∗∗∗

127.26±1.51^∗∗∗

114.42±3.31^∗∗∗

174.56±6.61^NS

166.36±10.95^NS

149.26±2.55^NS

Mucin Content (μg of alcian blue/g wet tissue)

110.2±1.31

61.28±2.22^a

64.49±1.61^NS

71.27±1.41^NS

75.48±2.15^∗∗

77.27±3.16^∗∗

74.83±2.41^∗

81.67±2.97^∗∗∗

86.26±3.73^∗∗∗

58.28±2.63^NS

68.84±2.01^NS

75.28±2.28^∗

Total Acidity (meq/L/4 hr)

1088.28 ± 22.29

1939.21 ±  42.71^b

1195.26 ± 37.03

1844.82 ± 28.77^NS

1775.82 ± 29.26*

1533.62 ± 15.72**

1545.82 ± 40.83**

1340.32 ± 41.94**

1226.42 ± 38.14**

2070.42±22.49^NS

1787.42±73.38^NS

1648.82 ± 29.64**

Total acid Output (meq/L/4 hr)

2459.08 ± 81.97

9318.16 ± 328.23^b

3068.32 ± 103.24

8398.11±428.97^NS

7280.47 ± 708.72**

6180.48 ± 172.84**

7574.04 ± 539.46*

5671.92 ± 308.91**

4741.44 ± 41.17**

7871.67±451.58^NS

7835.26±414.12^NS

7612.26 ± 359.52*

𝑁=6 normal group= vehicle Na CMC 1% (10 ml/kg, p.o.).
Values are expressed as mean ± SEM. Control group was compared with normal group. Test and standard groups were compared with control group. Statistical comparison was performed using analysis of variance (ANOVA) followed by Turkey’s test. ^a𝑃<.001; ^b𝑃<.01, when compared with normal group. *𝑃<.05; **𝑃<.01; ***𝑃<.001; NS: nonsignificant, when compared with control groups.
ME100, 200, and 400—methanolic extract of Rubia cordifolia 100, 200, and 400 mg/kg, p.o,. respectively, CF50,100, and 200—chloroform fraction of Rubia cordifolia 50, 100, and 200 mg/kg, p.o., respectively, RF50,100, and 200—residual fraction of Rubia cordifolia 50, 100, and 200 mg/kg, p.o., respectively.