Suppression of Heregulin-β1/HER2-Modulated Invasive and Aggressive Phenotype of Breast Carcinoma by Pterostilbene via Inhibition of Matrix Metalloproteinase-9, p38 Kinase Cascade and Akt Activation
Figure 2
Effect of pterostilbene on HRG-β1-mediated human breast cancer cell invasion. Invasion of tumor cells was analyzed in Transwell Boyden chambers with a polycarbonate filter of 8-m pore size. Serum-starved MCF-7 cells were seeded in Transwell upper chamber coated with a 1 : 80 diluted Matrigel. After 12 h attachment, cells plated in upper chamber were treated with HRG-β1 (20 ng/ml) in the presence of the indicated concentrations of pterostilbene and allowed to invade for 72 h. Invading cells on the lower surface of filter were stained and quantified with microscope. For quantification, the number of invasive cells were counted in 10 random selective fields per well and each bar represents mean ± SE of three independent experiments. ** and *** compared with HRG-β1-stimulated group; ### compared with serum-deprived control. PTER represents pterostilbene.
