Hypericum triquetrifolium—Derived Factors Downregulate the Production Levels of LPS-Induced Nitric Oxide and Tumor Necrosis Factor-α in THP-1 Cells

Figure 5

Hypericum triquetrifolium extract suppresses LPS-induced TNF-α gene expression in human THP-1 cells. RNAs from LPS-treated, extract-treated or LPS plus extract-treated THP-1 cells were reverse transcribed and amplified with human G3PDH and TNF-α primer pairs and electrophoresed. G3PDH and TNF-α messages amplified at around 983 and 325 bp, respectively. Extract demonstrated a significant suppression of endogenous TNF-α message (lane D) compared with that in the LPS-treated culture (lane B). These results are from a single experiment, which was repeated three times.