Hypericum triquetrifolium—Derived Factors Downregulate the Production Levels of LPS-Induced Nitric Oxide and Tumor Necrosis Factor-α in THP-1 Cells

Figure 6

Hypericum triquetrifolium extract suppresses LPS-induced iNOS gene expression in human THP-1 cells. RNAs from LPS-treated, extract-treated or LPS plus extract-treated THP-1 cells were reverse transcribed and amplified with human G3PDH and iNOS primer pairs and electrophoresed. Extract demonstrated complete suppression of endogenous iNOS message (lane D) compared with that in the LPS-treated culture (lane B). These results are from a single experiment, which was repeated three times, using THP-1 cells.