Insulin-Mimetic Action of Rhoifolin and Cosmosiin Isolated from Citrus grandis (L.) Osbeck Leaves: Enhanced Adiponectin Secretion and Insulin Receptor Phosphorylation in 3T3-L1 Cells
Figure 3
Effect of rhoifolin and cosmosiin on the adiponectin secretion from 3T3-L1 adipocytes. Nine days after the initiation of differentiation, cells were treated with control medium or test compounds for 24 h at 37°C. (a) Control medium (C); 10 nM insulin (I); rhoifolin 0.01 μM (lane 1), 0.1 μM (lane 2), 0.5 μM (lane 3), 1 μM (lane 4), and 5 μM (lane 5). (b) Control medium (C); 10 nM insulin (I); cosmosiin 1 μM (lane 1), 10 μM (lane 2), and 20 μM (lane 3). Secreted adiponectin was quantified by western blotting with antibody. The blots shown are one representative of three independent experiments. Values are means ± S.D. of three experiments and ; versus control.
