Insulin-Mimetic Action of Rhoifolin and Cosmosiin Isolated from Citrus grandis (L.) Osbeck Leaves: Enhanced Adiponectin Secretion and Insulin Receptor Phosphorylation in 3T3-L1 Cells
Figure 4
Effect of rhoifolin and cosmosiin on the phosphorylation level of insulin receptor-β. Nine days after the initiation of differentiation, cells were treated with control medium or test compounds for 20 min at 37C, and then lysates were prepared. (a) Control medium (C); 10 nM insulin (I); rhoifolin 0.01 μM (lane 1), 0.1 μM (lane 2), 0.5 μM (lane 3), 1 μM (lane 4), and 5 μM (lane 5). (b) Control medium (C); 10 nM insulin (I); cosmosiin 1 μM (lane 1), 10 μM (lane 2), and 20 μM (Lane 3). Phosphorylation of IRβ was assessed by western blot with anti-phospho-IRβ antibody. Values are means ± SD of three experiments and ; compared with control.
