Suppression of Ongoing Experimental Arthritis by a Chinese Herbal Formula (Huo-Luo-Xiao-Ling Dan) Involves Changes in Antigen-Induced Immunological and Biochemical Mediators of Inflammation
Figure 2
Alteration of the T cell proliferative and cytokine responses of HLXL-treated arthritic rats. LNCs of arthritic rats harvested on d 7 after initiation of the daily feeding of HLXL or water were tested for their T cell proliferative (a) and cytokine response (b)–(d) to antigenic restimulation with Bhsp65 in vitro. Ova served as the control antigen. The results of the proliferation assay were expressed as mean stimulation index (SI) ± SEM. The cpm value for PPD was 9,240 (the control group) and 5,577 (HLXL group). For cytokines, the level of mRNA for IL-17 (b), IFN- (c), and IL-10 (d) was measured by qRT-PCR, normalized to HPRT expression, and compared with that of cells in medium. The results of a representative experiment ( per group) are shown. The data are expressed as “fold relative to cells in the medium”. (*, ; **, ).
