Safety Evaluation of Yukmijihwang-tang: Assessment of Acute and Subchronic Toxicity in Rats

Table 2

Hematological parameters of male and female rats on subchronic toxicity study.

	(a) Male				(b) Female
Parameter	Vehicle control	YMJ-500 mg	YMJ-1000 mg	YMJ-2000 mg	Vehicle control	YMJ-500 mg	YMJ-1000 mg	YMJ-2000 mg
WBC (×10^3/μL)	12.10±1.04	10.72±1.06	10.49±0.48	10.83±0.58	8.74±0.71	8.54±0.72	7.56±0.71	9.33±0.91
Reticulocytes (%)	2.1±0.1	2.3±0.2	2.0±0.1	2.3±0.1	1.9±0.1	1.7±0.1	1.9±0.1	2.3±0.1
Neutrophils (%)	14.46±1.39	18.57±1.82	21.65±1.76^∗∗	18.37±1.10	13.07±2.25	13.27±1.57	15.91±0.74	14.98±2.31
Lymphocytes (%)	77.8±1.6	74.3±1.9	71.0±1.7^∗∗	75.1±1.2	80.4±2.2	79.2±1.7	76.9±0.8	79.1±2.2
Eosinophils (%)	1.1±0.1	1.1±0.2	1.2±0.1	1.1±0.2	1.2±0.2	1.2±0.2	1.2±0.1	1.1±0.1
Monocytes (%)	4.7±0.4	4.5±0.3	4.5±0.3	3.6±0.3	3.6±0.3	4.6±0.7	4.0±0.3	3.3±0.2
Basophils (%)	0.7±0.1	0.7±0.1	0.6±0.1	0.7±0.1	0.7±0.1	0.7±0.1	0.9±0.1	0.7±0.1
LUC (%)	1.3±0.1	0.9±0.1	1.0±0.1	1.1±0.2	1.0±0.1	0.9±0.1	1.1±0.2	0.9±0.1
RBC (×10^6/μL)	8.63±0.16	8.65±0.13	8.70±0.14	8.66±0.11	8.19±0.09	8.20±0.13	8.38±0.11	7.92±0.09
Hemoglobin (g/dL)	15.7±0.4	16.1±0.1	16.1±0.3	16.4±0.1	16.1±0.3	16.0±0.3	16.3±0.2	15.9±0.2
Hmatocrit (%)	44.6±1.0	45.4±0.5	45.4±0.7	46.0±0.5	44.9±0.6	44.1±0.7	45.3±0.4	43.8±0.5
MCV (fL)	51.7±0.8	52.5±0.9	52.2±0.7	53.1±0.5	54.9±0.5	53.8±0.5	54.1±0.4	55.4±0.6
MCH (pg)	18.2±0.3	18.7±0.3	18.5±0.3	19.0±0.2	19.7±0.2	19.5±0.2	19.5±0.2	20.1±0.2
MCHC (g/dL)	35.2±0.1	35.5±0.2	35.4±0.2	35.8±0.2	35.9±0.2	36.3±0.2	36.0±0.3	36.3±0.3
Platelet (×10^3/μL)	1048±24	1034±36	1053±36	1065±49	1089±48	1080±48	1098±20	1051±38
PT (sec)	16.1±0.4	15.5±0.3	16.0±0.4	16.8±0.6	14.5±0.2	14.3±0.2	14.2±0.3	14.1±0.1
APTT (sec)	18.6±0.4	18.8±0.6	18.2±0.4	18.8±0.3	15.0±0.2	14.8±0.5	15.3±0.4	15.1±0.4

Values are mean ± SEM for 10 rats in each group. ∗∗, significant differences from the vehicle control (0) group (𝑃<.01). WBC: white blood cells; LUC: large unstained cells; RBC: red blood cells; MCV: mean corpuscular volume; MCH: mean corpuscular hemoglobin; MCHC: mean corpuscular hemoglobin concentration; PT: prothrombin time; APTT: activated partial prothrombin time.