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Evidence-Based Complementary and Alternative Medicine
Volume 2011, Article ID 690518, 8 pages
http://dx.doi.org/10.1093/ecam/nep105
Original Article

In Vitro and In Vivo Antibacterial Activity of Punica granatum Peel Ethanol Extract against Salmonella

1College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan, Jeonbuk, 570-749, Republic of Korea
2Department of Pharmacy, College of Pharmacy, Wonkwang University, Republic of Korea
3College of Medicine, Wonkwang University, Jeonbuk 570-749, Republic of Korea
4Department of Oriental Medicine Resources, Sunchon National University, Jeonnam 540-742, Republic of Korea
5Department of Oceanography, Kunsan National University, Jeonbuk 573-701, Republic of Korea

Received 10 December 2008; Accepted 8 July 2009

Copyright © 2011 Jang-Gi Choi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Punica granatum is commonly used in Korea as a traditional medicine for the treatment of pathogenic bacteria. In this study, we investigated the in vitro and in vivo antimicrobial activity of P. granatum peel EtOH extract (PGPE) against 16 strains of Salmonella. The minimal inhibitory concentrations of PGPE were in the range of 62.5–1000 x03BCg mL-1. In addition, the in vivo antibacterial activity of the PGPE extract was examined in a S. typhimurium infection mouse model. Mice were initially infected with S. typhimurium and then with PGPE. The extract was found to have significant effects on mortality and the numbers of viable S. typhimurium recovered from feces. Although clinical signs and histological damage were rarely observed in the treated mice, the untreated controls showed signs of lethargy and histological damage in the liver and spleen. Taken together, the results of this study indicate that PGPE has the potential to provide an effective treatment for salmonellosis.