Research Article
Antiosteoporotic Activity of Dioscorea alata L. cv. Phyto through Driving Mesenchymal Stem Cells Differentiation for Bone Formation
Figure 1
HPLC analysis of Dispo85E. (a) Reverse-phase HPLC profile. Column: Mightysil RP-18 (4.6 × 250 mm, 5 mm); mobile phase: methanol-water (methanol: 10–100% in 120 minutes); flow rate: 0.8 mL/minute; detection wavelength: 254 nm. (b) Normal-phase HPLC profile. Column: Cosmosil 5SL-II (4.6 × 250 mm, 5 mm); mobile phase: 100%–98% DCM in MeOH (0–15 minutes), 98%–95% DCM (15–25 minutes), 95%–90% DCM (25–35 minutes), 90%–80% DCM (35–45 minutes), and 80%–70% DCM (45–55 minutes); flow rate: 0.8 mL/minute; detection wavelength: 254 nm.
