Antiosteoporotic Activity of Dioscorea alata L. cv. Phyto through Driving Mesenchymal Stem Cells Differentiation for Bone Formation
Figure 3
Dispo85E stimulates osteoblastogenesis and inhibits adipogenesis in the mesenchymal stem cell line C3H10T1/2. C3H10T1/2 cells were grown to confluence in a standard medium. One day after confluence, the medium was replaced with a permissive osteogenic/adipogenic medium containing Dispo85E at the indicated concentrations. At PID 12, the mRNA level of IGF-1 (a) and OCN (b) was analyzed by real-time PCR (). (c) ALP activity and (d) the effect of Dispo85E on cell viability were measured (). (e) Cells were induced with adipogenic medium and treated with drugs. At PID 12, the cells were stained with oil red O to assess lipid accumulation (magnification, 200-fold). (f) After 16 days of culture in adipogenic medium, cells were harvested, fixed, and stained with Nile red solution. Percentage of Nile-red-stained cells in the total population of each sample were quantified with FACScan flow cytometry (). **Indicates significant difference from control group at ; *Indicates significance at .
