Nordihydroguaiaretic Acid from Creosote Bush (Larrea tridentata) Mitigates 12-O-Tetradecanoylphorbol-13-Acetate-Induced Inflammatory and Oxidative Stress Responses of Tumor Promotion Cascade in Mouse Skin
Figure 5
Effect of NDGA on TPA induced morphological changes as observed by H & E, 100. Representative pictures are shown. (a) Photomicrograph of skin section from acetone treated control animal showing normal skin structure having well defined epidermal and dermal layers. Epidermis is just 2-3 cells thick. Superficial dermis does not show any edema or PMN infiltration; (b) double dose of TPA treatment showing skin with irregular epidermal thickening, severe dermal edema and infiltration of PMNs in upper dermis; (c) mice treated with NDGA (15 μmol) before topical application of TPA showing moderate epidermal thickening moderate dermal edema and scattered dermal infiltrates; (d) section of skin treated with higher dose of NDGA (25 μmol) before TPA application showing mild epidermal thickening, occasional infiltration of leukocytes and mild edema; and (e) and (f) sections of skin from mice treated with NDGA 15 and 25 μmol, respectively, followed by acetone application showing normal skin structure. Detail of histological observations is also given in Table 1.
