Low-Frequency Electroacupuncture Improves Insulin Sensitivity in Obese Diabetic Mice through Activation of SIRT1/PGC-1𝛼 in Skeletal Muscle

Table 1

Primers used in PCR.

Gene	Primer sequence	Gene number	Product length
SIRT1 (mouse, rat)	Fw 5′-CAGTGTCATGGTTCCTTTGC-3′	AF214646	104 bp
	Rv 5′-CACCGAGGAACTACCTGAT-3′
PGC-1alpha (mouse, rat)	Fw 5′-ATGAATGCAGCGGTCTTAGC-3′	AF049330	174 bp
	Rv 5′-TGGTCAGATACTTGAGAAGC-3′
NRF1 (mouse)	Fw 5′-GGAGCACTTACTGGAGTCC-3′	NM010938	143 bp
	Rv 5′-CTGTCCGATATCCTGGTGGT-3′
ACOX (mouse)	Fw 5′-GGTGGTATGGTGTCGTACTTGA-3′	NM015729.2	296 bp
	Rv 5′-GAATCTTGGGGAGTTTATCTGC-3′
GAPDH (mouse, rat)	Fw 5′-GCCAAAAGGGTCATCATCTC-3′	BC082592	226 bp
	Rv 5′-GGCCATCCACAGTCTTCT-3′