Ethanol Extract of Chinese Propolis Facilitates Functional Recovery of Locomotor Activity after Spinal Cord Injury
Figure 2
EECP attenuated the expression of iNOS mRNA and enhanced that of BDNF and NT-3 mRNAs in the site of SCI. Vehicle or EECP (1 mg/kg) was daily administered to rats with SCI. The results of RT-PCR analysis of various genes in the injured spinal cord of rats that had received daily administration of vehicle or EECP are shown. (a) The expression level of iNOS mRNA in the injured spinal cord was significantly lower in the EECP-treated group () than in the vehicle-treated group () on experimental days 1 and 3. (b), (c) and (d) mRNA expression levels of BDNF (b) and NT-3 (c) were significantly higher in the EECP-treated group () than in the vehicle-treated group () on experimental day 7, but there was no significant difference in the GDNF (d) mRNA level between the 2 groups (). Significance of differences from vehicle-treated group was determined by using two-way ANOVA followed by the Bonferroni post-test. , versus vehicle-treated group. -Actin was used as the internal control.
