Evodia rutaecarpa and Three Major Alkaloids Abrogate Influenza A Virus (H1N1)-Induced Chemokines Production and Cell Migration
Figure 3
Concentration-related cell migration of HL-60-differentiated macrophages in response to RANTES (a) and MCP-1 (b). Cells were placed in the upper well and chemokines were added into the lower wells of the chamber for the assessment of migration activity. The entire chamber was then incubated for 6 h at 37°C. Migration was assessed by counting migrated cells in five microscopic fields per well at 400× magnification. Data reported are mean ± SE of six independent experiments, each performed in triplicate. *P .05 and **P .01 indicate degrees of statistical significance of difference as compared to un-stimulated cells.
