Evodia rutaecarpa and Three Major Alkaloids Abrogate Influenza A Virus (H1N1)-Induced Chemokines Production and Cell Migration
Figure 4
Effects of ER (a) and three major alkaloids (b) on RANTES-induced migration of HL-60-differentiated macrophages. Cells pre-incubated with respective tested agents for 30 min were plated into the upper wells of the chamber. RANTES (100 ng m) was then added to the lower wells to induce cell migration for 6 h. Migration was assessed by counting migrated cells in five microscopic fields per well at 400× magnifications. RANTES-induced cell migration minus spontaneous migration in PBS served as control and was designated as 100. Data reported are mean ± SE of six independent experiments, each performed in triplicate. *P .05 and **P .01 indicate degrees of statistical significance of difference as compared to samples without tested agents treatment.