Evodia rutaecarpa and Three Major Alkaloids Abrogate Influenza A Virus (H1N1)-Induced Chemokines Production and Cell Migration
Figure 5
Effects of ER (a) and three major alkaloids (b) on MCP-1-induced migration of HL-60-differentiated macrophages. Cells pre-incubated with respective tested agents for 30 min were plated into the upper wells of the chamber. MCP-1 (100 ng m) was then added to the lower wells to induce cell migration for 6 h. Migration was assessed by counting migrated cells in five microscopic fields per well at 400× magnifications. MCP-1-induced cell migration minus spontaneous migration in PBS served as control and was designated as 100. Data reported are mean ± SE of six independent experiments, each performed in triplicate. *P .05 and **P .01 indicate degrees of statistical significance of difference as compared to samples without tested agents treatment.
