Anticancer Potential of Aqueous Ethanol Seed Extract of Ziziphus mauritiana against Cancer Cell Lines and Ehrlich Ascites Carcinoma
Figure 2
Flow cytometric analysis of ZMS-induced apoptosis and necrosis in HL-60 cells using annexinV-FITC and PI double staining. HL-60 cells (1 × 106 /mL) were incubated with indicated concentrations of ZMS for 12 hours and stained with Annexin V-FITC/PI. Quadrant analysis of fluorescence intensity of ungated cells in FL-1 versus FL-2 channels was from 10000 events. Cells in the lower right quadrant represented apoptosis while in the upper right quadrant indicated postapoptotic necrosis and representative of one of the three similar experiments.
