Combination of Medicinal Herbs KIOM-79 Reduces Advanced Glycation End Product Accumulation and the Expression of Inflammatory Factors in the Aorta of Zucker Diabetic Fatty Rats

Figure 3

The effects of KIOM-79 treatment on expression of MCP-1, VEGF, and VCAM-1 in the aorta. Representative immunohistochemistry images for MCP-1 (A), VEGF-1 (B), and VCAM-1 (C) staining in the aorta of Zucker lean rats ((a), (e), and (i)), untreated ZDF rats ((b), (f), and (j)), ZDF rats treated with AG (50 mg/kg) ((c), (g), and (k)), and ZDF rats treated with KIOM-79 (50 mg/kg) ((d), (h), and (l)). Original magnification: ×400. After real time-PCR analysis, MCP-1 (D), VEGF (E), and VCAM-1 (F) levels were normalized to GAPDH. All data are expressed as mean ± S.E.M. *P <  .01 compared with ZL rats; ^#P <  .05 compared with untreated ZDF rats.