Combination of Medicinal Herbs KIOM-79 Reduces Advanced Glycation End Product Accumulation and the Expression of Inflammatory Factors in the Aorta of Zucker Diabetic Fatty Rats

Figure 4

The effects of KIOM-79 treatment on activation of NF-κB and iNOS in the aorta. Representative NF-κB (A) and iNOS (B) staining in the aorta in Zucker lean rats ((a), (e)), untreated ZDF rats ((b), (f)), ZDF rats treated with AG (50 mg/kg) ((c), (g)), and ZDF rats treated with KIOM-79 (50 mg/kg) ((d), (h)). NF-κB staining was observed in the nuclei of both the endothelial layer and smooth muscle lesion (arrow), and iNOS staining (brown color) was observed in the aorta. Hematoxylin counterstain. Original magnification: ×400. Analysis of EMSA of NF-κB activation (C) and Western blot of iNOS (D). Quantitative analyses of the NF-κB and iNOS stains were completed. All data were expressed as mean ± S.E.M. *P <  .01 compared with ZL rats; ^#P <  .05 compared with untreated ZDF rats.