Role of Bone Morphogenetic Proteins-7 (BMP-7) in the Renal Improvement Effect of DangGui (Angelica sinensis) in Type-1 Diabetic Rats
Figure 6
Effect of DangGui on free radicals in glucose-exposed RMCs. (a) Direct free radical scavenging activity of DangGui was determined by the DPPH radical scavenging assay. Values represent the data of three independent experiments (SE < 0.05). Ascorbic acid was used as a reference compound. (b) DHE stains were used to visualize the intracellular ROS in RMCs. (c) Lucigenin assays were used to quantitate the generation of superoxide in RMCs. RMCs were cultured in normal glucose (5 mmol/L; control) or high glucose (30 mmol/L; HG) medium in the presence of DangGui (200 μg/mL) or tiron (10 μmol/L) for 24-h incubation. *P < 0.05 compared to control, #P < 0.05 compared to HG.
