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Evidence-Based Complementary and Alternative Medicine
Volume 2011, Article ID 824104, 7 pages
http://dx.doi.org/10.1155/2011/824104
Research Article

Bacillus amyloliquefaciens G1: A Potential Antagonistic Bacterium against Eel-Pathogenic Aeromonas hydrophila

1Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources of Ministry of Education, Aquatic Pathogen Collection Center of Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China
2Education Department, Shanghai Normal University, Shanghai 200235, China
3Technology Department, Shanxi Veterinary Drug and Feed Engineering Technology Research Center, Yuncheng 044000, China

Received 24 December 2010; Accepted 16 May 2011

Copyright © 2011 Haipeng Cao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Recent studies have revealed that the use of probiotics is an alternative to control marine aeromonas. However, few probiotics are available against Aeromonas hydrophila infections in eels. In the present study, a potential antagonistic strain G1 against the eel-pathogenic A. hydrophila was isolated from sediment underlying brackish water. Its extracellular products with antibacterial activities were shown to be stable under wide range of pH, temperature, and proteinase K. It was initially identified as Bacillus amyloliquefaciens using API identification kits and confirmed to be B. amyloliquefaciens strain (GenBank accession number DQ422953) by phylogenetic analysis. In addition, it was shown to be safe for mammalians, had a wide anti-A. hydrophila spectrum, and exhibited significant effects on inhibiting the growth of the eel-pathogenic A. hydrophila both in vitro and in vivo. To the best of our knowledge, this is the first report on a promising antagonistic Bacillus amyloliquefaciens strain from brackish water sediment against eel-pathogenic A. hydrophila.