Agaricus blazei Extract Induces Apoptosis through ROS-Dependent JNK Activation Involving the Mitochondrial Pathway and Suppression of Constitutive NF-κB in THP-1 Cells
Figure 1
Effect of ABE on cell viability and cell death in
THP-1 cells. THP-1 cells were seeded at 2 × 105 cells ml−1 and
were then treated with ABE at the indicated concentrations for 24 h. (a)
Cell viability was determined by MTT assay. Each point represents the mean ± SD of three
independent experiments. The significance was determined by Student's t-test
( versus vehicle control (0)). (b) DNA contents and
(c) annexin-V+ cells were analyzed by flow cytometer. Percentages
represents the sub-G0/G1 phase and annexin-V+ cells, respectively.
The results shown are from one representative experiment of three experiments that exhibited similar
patterns.
