Effect of Supplementation of Tanshinone IIA and Sodium Tanshinone IIA Sulfonate on the Anticancer Effect of Epirubicin: An In Vitro Study
Figure 2
Effect of Tan IIA or STS on epirubicin-induced cytotoxicity and apoptosis in BT-20 breast cancer cells. (a) BT-20 breast cancer cells were treated with 0–2 μg/mL epirubicin in the presence or absence of STS (0–50 μg/mL) or Tan IIA (0–50 μg/mL) for 72 h. The cell growth was determined by WST-1 assay. (b) BT-20 cells were treated with 1 μg/mL of epirubicin. The change in apoptosis with the addition of STS (50 μg/mL) and Tan IIA (2, 10, 25, 50 μg/mL) in epirubicin- (Epi-) treated BT-20 cells was determined by flow cytometry at 48 or 72 h. * when compared with the control group; + when compared with the epirubicin group. Data are the mean ± SD of triplicates from a representative assay of three separate experiments.
