Antinociceptive Activities and the Mechanisms of Anti-Inflammation of Asiatic Acid in Mice
Figure 7
Inhibition of iNOS, COX-2, and NF-κB protein expression by AA induced by Carr in mice paw edema for 5 h. Normal control received 0.9% normal saline. Animals treated with AA (1, 5, and 10 mg/kg) and Indo to injection of Carr right hind paws. The right hind paw tissues were taken at the 5th h. Then, the homogenate was centrifuged and tissue suspended and were then prepared and subjected to western blotting using an antibody specific for iNOS, COX-2, and NF-κB. β-actin was used as an internal control. (a) Representative western blot from two separate experiments is shown. (b) Relative iNOS, COX-2 and NF-κB protein levels were calculated with reference to Carr-injected mice. ###compared with sample of control group. The data were presented as mean ± S.D. for three different experiments performed in triplicate.** and *** were compared with Carr-alone group.
