A Preclinical Evaluation of Antrodia camphorata Alcohol Extracts in the Treatment of Non-Small Cell Lung Cancer Using Non-Invasive Molecular Imaging
Figure 4
In vitro inhibition of migration/invasiveness of H441GL cells by ACAE. (a) Wound-scratch assay data revealed that ACAE exerted a dose-dependent inhibitory effect on H441GL invasive ability after 24 h treatment period. Bar, 500 μm (left). Quantitatively, at 150 μg mL-1 of ACAE, a 60% of motility inhibition was observed (right, *P < .05; **P < .01). (b) Semi-quantitative analysis of ACAE-treated H441GL cell lysate demonstrated that CXCR4 transcript level (left) was down-regulated at 150 μg mL-1 of ACAE. The lower part represents data expressed quantitatively by densitometric means. Western blot analysis of ACAE-treated H441GL cell lysate probed with anti-CXCR4 antibody (right). CXCR4 expression was down-regulated by ACAE treatment in a concentration-dependent manner. When represented quantitatively (lower), 150 μg mL-1 of ACAE exhibited the greatest suppression of CXCR4 expression (**P < .01). (c) Invasive ability of H441GL cells was suppressed by ACAE treatment as demonstrated by transwell analysis (left, **P < .01), while matrix metallo-protease (MMP) zymography demonstrated (left) that both pro-MMP9 (92 kD) and pro-MMP2 (72 kD) enzymatic activity in H441GL cells was suppressed by ACAE in a dose-dependent manner.
