8-Prenylkaempferol Suppresses Influenza A Virus-Induced RANTES Production in A549 Cells via Blocking PI3K-Mediated Transcriptional Activation of NF-κB and IRF3
Figure 2
Time-related effect of H1N1 inoculation on (a) transcriptional factors activation measured by NF-B and IRF-3 nuclear translocation, IB degradation and c-Jun phosphorylation, as well as (b) Akt phosphorylation in A549 alveolar epithelial cells, respectively. Nuclear (for NF-B and IRF-3) and cytosolic proteins (for IB and c-Jun) were extracted in the absence of virus (indicated as 0) or obtained at 1, 6, or 16 h p.i as described in Section 2 then assessed by western blotting. Similar results were obtained in four independent experiments.
