8-Prenylkaempferol Suppresses Influenza A Virus-Induced RANTES Production in A549 Cells via Blocking PI3K-Mediated Transcriptional Activation of NF-κB and IRF3

Figure 4

Effects of wortmannin on NF-𝜅B and IRF-3 nuclear translocation, I𝜅B degradation and Akt phosphorylation in H1N1-infected A549 cells. For nuclear translocation and cytosolic I𝜅B degradation, cells were lysed at 16 h p.i. and then protein was extracted for western blot analysis as described in Section 2. While assessing Akt phosphorylation, cells were lysed at 6 h p.i. then cytosolic protein was extracted. Similar results were obtained in three independent experiments.

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