Original Article

Anti-Obesity and Anti-Diabetic Effects of Acacia Polyphenol in Obese Diabetic KKAy Mice Fed High-Fat Diet

Figure 2

Representative histological sections of liver stained with HE (a) or oil red O (b). KKAy mice were given a LF, a HF, HF containing 2.5% AP (HF/AP 2.5%) or HF containing 5.0% AP (HF/AP 5.0%) for 7 weeks. The liver was removed, fixed in 10% neutral-buffered formalin, paraffinized and stained using HE (a). A cryostat was also used to prepare tissue sections for staining with oil red O (b). Details of the diets are given in the “Materials and Methods” section.
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(a) HE Staining
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(b) Oil red O staining