Anti-Obesity and Anti-Diabetic Effects of Acacia Polyphenol in Obese Diabetic KKAy Mice Fed High-Fat Diet
Table 2
Body weight gain, and plasma and hepatic biochemistry of mice fed on the experimental diets for 7 weeks.
LF
HF
HF/AP 2.5
HF/AP 5.0
Food intake (g/mouse/day)
5.1 ± 0.9
4.5 ± 0.6
4.6 ± 0.5
4.4 ± 0.5
Energy intake (kcal/mouse/day)
19.4 ± 3.6
23.3 ± 3.2*
23.5 ± 2.8*
22.0 ± 2.9*
Pre-diet body weight (g)
31.3 ± 1.2
30.9 ± 1.1
31.5 ± 1.6
31.1 ± 1.0
Post-diet body weight (g)
39.7 ± 2.4
51.4 ± 2.5**
45.7 ± 4.7**,##
39.5 ± 1.6##
Body weight gain (g)
8.4 ± 1.9
20.5 ± 2.0**
14.2 ± 4.3**,##
8.4 ± 1.9##
Whole WAT (g)
3.14 ± 0.60
4.66 ± 0.34**
4.00 ± 0.35**,##
3.37 ± 0.54##
Epididymal WAT (g)
1.63 ± 0.23
1.97 ± 0.21*
1.77 ± 0.22#
1.70 ± 0.22#
Retroperitoneal WAT (g)
0.96 ± 0.28
1.57 ± 0.21**
1.31 ± 0.19**
0.94 ± 0.19##
Perirenal WAT (g)
0.66 ± 0.14
1.35 ± 0.19**
0.91 ± 0.19*,##
0.76 ± 0.23##
Liver weight (g)
1.85 ± 0.22
3.27 ± 0.44**
1.83 ± 0.40##
1.26 ± 0.13**,##
Liver triglyceride (mg liver)
78.0 ± 13.8
156.5 ± 11.9**
82.2 ± 4.5##
39.5 ± 6.1**,##
Liver cholesterol (mg liver)
2.3 ± 0.4
4.4 ± 0.7**
1.0 ± 0.6**,##
0.9 ± 0.4**,##
Glucose concentration (mmol )
6.7 ± 2.1
20.8 ± 4.4**
8.4 ± 3.2##
5.6 ± 1.5##
Insulin concentration (U m)
22.7 ± 16.5
171.5 ± 120.9**
42.7 ± 33.4##
28.8 ± 13.0##
HOMA-IR
7.5 ± 3.9
139.9 ± 81.6**
20.6 ± 17.3##
7.7 ± 5.4##
GOT concentration (U )
42.7 ± 14.8
176.3 ± 77.9**
82.2 ± 59.5#
64.8 ± 29.6#
GPT concentration (U )
16.8 ± 5.0
56.7 ± 14.0**
12.2 ± 3.0##
19.8 ± 7.8##
KKAy mice were given a low-fat diet (LF), a high-fat diet (HF), HF containing 2.5 AP (HF/AP 2.5) or HF containing 5.0 AP (HF/AP 5.0) for 7 weeks. After measuring body weight, white adipose tissue and liver were removed and weighed, and levels of liver triglyceride and cholesterol were measured. Blood samples were also collected to measure glucose, insulin, GOT and GPT levels. HOMA-IR was calculated from glucose and insulin levels after overnight fasting. Food intake was measured once weekly. Details of the diets are given in the “Materials and Methods” section. Data represent means ± SDs of 10 mice per group. Tukey's test: *P .05 versus LF; **P .01 versus LF; #P .05 versus HF; ##P .01 versus HF.
