Seed Oil of Brucea javanica Induces Apoptotic Death of Acute Myeloid Leukemia Cells via Both the Death Receptors and the Mitochondrial-Related Pathways

Figure 8

Active contents of Brucea Javanica oil. (a) HL-60 and U937 cells were treated with oleic acid (37.5, 75, and 150 μg/mL) as indicated for 6 h. (b) HL-60 and U937 cells were treated with linoleic acid (56.25, 112.5, 225 μg/mL) as indicated for 6 h. (c) HL-60 and U937 cells were treated with the mixture of oleic acid and linoleic acid as the the same ratio as in BJO (low:oleic acid 37.5 μg/mL & linoleic acid 56.25 μg/mL; Middle: oleic acid 75 μg/mL & linoleic acid 112.5 μg/mL; High: oleic acid 150 μg/mL & linoleic acid 225 μg/mL) for 6 h. (d) U937 and HL-60 cells morphology analysis following treatment with the mixture of oleic acid and linoleic acid. AO/EB was used to stain cells and the cellular fluorescent changes were observed using fluorescence microscope. Results were obtained from three separate experiments.