965153.fig.003a
(a)
965153.fig.003b
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965153.fig.003c
(c)
Figure 3: Tissue distribution of α-globin and β-globin mRNA. (a) Northern blot showing tissue distribution and size of the P. flesus   α1-globin and β1-globin transcripts. (b) Relative mRNA expression levels of α1-globin and β1-globin in different tissues. α-globin and β-globin expressions in different tissues were analyzed by real-time qPCR with 18S rRNA as reference gene. Values are relative fold change with brain as 1 for pooled samples from 8 SW-acclimated adult flounder; (c) expression levels of four types of globin mRNAs in kidney. α-globin and β-globin expressions in kidney were analyzed by real-time qPCR with 18S rRNA as reference gene. Values are relative fold change with α2-globin as 1 for pooled samples from 8 SW-acclimated adult flounder.