Figure 4: Relative mRNA expression levels of four types of globin and concentration of NOx in kidney of FW- and SW-adapted flounder. (a) Expressions of four types of globin were analyzed by real-time qPCR with β-actin as reference gene. Values are relative fold change with α2-globin in SW-adapted flounder kidney as 1; the significant difference between FW- and SW-adapted flounder was indicated as * ( ). (b) Concentration of total NO in kidney. Values are measurements from 7 pooled kidneys of FW- or SW-adapted flounder.